| STUDY TITLE: | Positron Emission Tomographic (PET) Scanning of Sympathetic Innervation and Function in Patients with Neurocardiologic Disorders | ||
| INSTITUTE: | National Institute of Neurological Disorders and Stroke | ||
| STUDY NUMBER: | 94-N-186 | ||
| PRINCIPAL INVESTIGATOR: | David S. Goldstein, MD, Ph.D. | ||
The existence and importance of the sympathetic nervous system have been known for many years. The difficulty has been in seeing it and measuring its activity. This is because the system consists of millions of tiny nerve endings in the heart and other organs as well as in mesh-like networks in the walls of blood vessels. In this study, we should be able to visualize the location and function of sympathetic nerves in the various organs of the body, to determine the effects of various drugs or stressors on the system, and to suggest how the activity of the system may change during various disorders.
We are asking you to participate in this study because you may have one of the following disorders: (1) clinical findings such as chest pain that suggest coronary artery disease; (2) decreased heart function; (3) high blood pressure; or (4) known or suspected abnormalities of the autonomic nervous system. which is a general term to describe the portion of the nervous system involved with involuntary activities of glands and internal organs.
All these conditions may involve abnormal function of the sympathetic nervous system in the heart.
A positron emitter is a type of radioactive substance that releases a form of radiation that can penetrate the body and reach detectors outside it, enabling construction of a "PET scan" Other "scans" used in nuclear medicine use a somewhat different source of radioactivity, but the idea is about the same.
Fluorodopamine is a drug that is structurally similar to the biochemicals of the sympathetic nervous system, noradrenaline and adrenaline. Just as some radioactive chemicals get taken up by bone, producing a bone scan, or get taken up by the brain, producing a brain scan, fluorodopamine gets taken up into sympathetic nerve endings, and the result is a scan of the sympathetic nervous system. For instance, we know that fluorodopamine gets taken up very readily into the heart walls, because there are so many sympathetic nerve endings there. Because there are so many sympathetic nerve endings in the heart, PET scans of the heart after injection of fluorodopamine basically look like images of the heart itself.
You may be asked to discontinue medications, such as for high blood pressure, for two weeks before the testing. Do not discontinue any medications before you or your doctor discuss this with Dr. Goldstein, the Principal Investigator for this study, or one of the cardiologists who are Associate Investigators for this study. If it is decided that discontinuing your medications would be unsafe, then you may be excluded from the study.
You will not be paid for participating in this study, because you are likely to benefit personally from the information provided by the study, you are being followed at an NIH clinic, or you are receiving prescription drugs through the NIH.
A typical PET scanning session takes place over the course of most of a morning and into the afternoon.
After you urinate to empty your bladder, electrocardiographic (EKG) leads will be attached to your chest, in order to monitor your heart rate and rhythm. Then you will undergo insertion of one or possibly two intravenous ("i.v.") catheters and probably an arterial catheter in the elbow area. The arterial catheter is described in detail below.
You will then be brought into the PET scanning room and lay down on an X-ray type table, which slides you into the hole of the doughnut-shaped scanner. You will be in the scanner for a few hours, and you may become bored. If you wish, you may bring a portable cassette recorder and listen to quiet music via earphones during the testing session.
In most cases, the chest will be in the PET scanner. In cases where we are evaluating an arm rather than the he art, your arm would be in the scanner, or a device like a Geiger counter will be used to record total radioactivity in an area of the limb, without PET scanning. In a few cases, the abdomen rather than the chest will be in the scanner.
An arterial catheter looks like a regular i.v. tube, and it is inserted in about the same place-the elbow crease area or sometimes the wrist area--but the tube is inserted into an artery, not a vein. Arteries carry the blood pressure, and if an arterial catheter is in place, we can measure your blood pressure continuously. The main other reason for inserting an arterial catheter as part of this testing is to enable us to draw blood samples rapidly, repeatedly, and without causing you pain. Because of the high pressure, and the fact that arteries cannot be seen in the skin, certain special procedures are involved. First, the skin is numbed with a local anesthetic, so that you don't feel pain when the catheter is inserted. Second, every so often the catheter will be flushed with a dilute anticoagulant solution, which prevents clogging of the catheter with a blood clot. Third, after the catheter is removed, local pressure will be applied for 10 minutes to prevent bleeding under the skin. And fourth, the area will be clean and the catheter will be in for a minimal period of time, to prevent infection. None of these complications, or any other serious complication, has occurred in over 150 arterial catheter insertions we have done since about 1980.
The patch of skin where the arterial catheter will be inserted will be numbed with lidocaine, a "caine" type of local anesthetic. The catheter at first surrounds an even thinner needle. After the needle is in the artery' the tube is passed over the needle into the artery and the needle removed, so that all that is left in the artery is the plastic tube. The arterial catheter is about 2 inches long. You should feel either nothing or else a slight pressure when the catheter is placed. A very similar plastic tube will be placed in an arm vein; the i.v. will be used to inject the fluorodopamine.
After the i.v. catheter is in place, fluid will be passed through at a slow rate.
After about 20 minutes of rest, baseline blood samples will be drawn from the arterial catheter.
A preliminary "attenuation scan" will be obtained. The attenuation scan, which lasts several minutes, enables us to check whether the heart (or other organ of interest) is positioned correctly in the field of the scanner's view. The attenuation scan is also needed for appropriate analysis of the scanning results after injection of the fluorodopamine.
Positron-emitting fluorodopamine will then be injected during an interval of a few minutes. In most cases, the injection will be i.v. You should not feel anything unusual due to the fluorodopamine injection. After the injection, PET scanning will be done for up to 3 hours. It is possible that another scan will be obtained after this period-either another attenuation scan or a scan after injection of a perfusion imaging agent (described below).
Some people have an urge to urinate after lying down for a few hours. If you feel an urge to urinate during the PET scanning but are unable to do so, you may have relief by having the urine drained using a condom or bladder catheter. This would only be done at your request and for your comfort. If a bladder catheter is used, then your urine will be cultured to test for infection. At the end of the testing session, the vascular catheters will be removed, and after we have checked to make sure there has been no bleeding under the skin, you will urinate into a container in order for us to determine how much radioactivity already is in the urine.
After the PET scanning session, you will stay overnight in the Clinical Center for observation and to collect all your urine for 24 hours from the time of injection of the fluorodopamine.
Yohimbine You may receive yohimbine by vein (a quick injection followed by a slow infusion). This drug stimulates release of noradrenaline (norepinephrine) from sympathetic nerves. Yohimbine often causes trembling, goosebumps, and cool, clammy palms. We have infused yohimbine many times in other clinical protocols, without any permanent untoward effects. Some patients have reported emotional feelings, such as elation, anxiety, or depression, but most have noted nothing unusual. Yohimbine may increase your blood pressure temporarily. In the event of an extreme increase in blood pressure (which can occur in hypertensive patients), an antidote anti-hypertensive drug, clonidine, will be available for use. In the 7 years during which we have used yohimbine us a research drug, in studies involving about 100 subjects. only 1 patient has required the antidote. The infusion will be given during most or all of the PET scanning.Trimethaphan Trimethaphan is a type of drug called a ganglion blocker. It interferes with transmission of sympathetic nerve impulses through way-stations between the brain and the rest of the body. Trimethaphan can cause a dry mouth, dizziness and low blood pressure during standing, and decreased urinary and bowel movements. The drug is rapidly inactivated and must be given continuously by intravenous infusion. Its effects wear off very rapidly after the infusion is stopped. You will receive trimethaphan at a dose that produces characteristic effects on blood pressure and pulse rate responses during simple maneuvers such as blowing against a resistance for a few seconds. The infusion will be given during most or all of the PET scanning. We have infused trimethaphan many times in other clinical protocols, without any serious side effects. Your blood pressure is likely to fall slightly; if it falls excessively, the infusion rate will be adjusted, or the infusion may be stopped. The investigator will have available an appropriate antidote vasoconstrictor drug; however, we have never had to use it. You will not receive trimethaphan if you have a particular kind of glaucoma, an eye disease.
Nitroprusside Nitroprusside is a type of drug called a vasodilator. Nitroprusside relaxes blood vessels, causing blood pressure to fall. The fall in blood pressure activates the sympathetic nervous system reflexively, and the sympathetic activation tends to restore the blood pressure. Nitroprusside is therefore used to test the ability of the sympathetic nervous system to react appropriately. Nitroprusside is a powerful drug that works very rapidly. The drug is given i.v., and after stopping a nitroprusside infusion, the effects of the drug disappear rapidly. If you receive Nitroprusside, the drug will be given at a carefully controlled rate i.v. using a programmed infusion pump. Your blood pressure will be monitored closely and continuously by an investigator. The infusion rate will be adjusted so that your blood pressure decreases by 5-10 millimeters of mercury (about 5-10%). During the infusion, you should note nothing special. If the blood pressure begins to fall beyond the criterion amount, the infusion rate will be adjusted, or the infusion may be stopped. The investigator will have available an appropriate antidote vasoconstrictor drug; however, we have never had to use it. We have infused nitroprusside many times in other clinical protocols, without any untoward effects. The infusion will be given during most or all of the PET scanning.
Desipramine You may receive i.v. or orally a drug called desipramine, before the PET scanning. Desipramine is used commonly in psychiatry to treat depression; to produce an anti-depressant effect, however, the drug must be given many times over about two weeks. The effects of one or two doses of desipramine are remarkably few--perhaps fatigue or dry mouth. Other side effects can include dizziness or a fast pulse rate when standing. We are including desipramine because it interferes with uptake of fluorodopamine into sympathetic nerve endings, and interference with the picture of the heart in subjects pretreated with desipramine would demonstrate that the picture does in fact portray the sympathetic nerves. We have administered desipramine many times in other clinical protocols, without any untoward effects.
Methylprednisolone Methylprednisolone acetate is a "steroid.. There are many metabolic effects of steroids. We are using this drug to inhibit the uptake of positron-emitting fluorodopamine by cells that are not nerves. Although chronic administration of this type of drug produces many medical and psychiatric effects, a single injection should be safe.
Tyramine Tyramine is a chemical found in some foodstuffs such as hard cheese and wine. When given as a drug, tyramine releases norepinephrine, the chemical messenger of the sympathetic nervous system. Tyramine produces no direct effects, but because of the norepinephrine release, tyramine usually increases blood pressure. The drug works rapidly, so that it is given by i.v. infusion, and the effects of the drug disappear soon after the infusion ends. You will receive tyramine to increase your blood pressure by 10-20 millimeters of mercury. This amount of increase in blood pressure corresponds to that occurring during mild exercise. The infusion will be given during most or all of the PET scanning. Tyramine has been used in diagnostic testing for conditions such as autonomic failure and pheochromocytoma, in order to assess the presence of releasable stores of norepinephrine. If you have a pheochromocytoma, you will not receive tyramine, because the drug can stimulate the tumor and produce dangerously high blood pressure. Tyramine also is contraindicated in patients receiving treatment with some types of anti-depressant medication.
Sedative In patients with some forms of heart disorders, sedatives are often prescribed. Although it is likely that sedation affects the function of the sympathetic nerves in the heart, this issue has never been addressed directly in people. You may receive a sedative drug, such as alprazolam, orally before the PET scanning; or you may receive the rapidly-acting sedative, diazepam, i.v. If you are given a sedative, appropriate monitoring of your pulse rate, blood pressure, and breathing will be done. We have used alprazolam or diazepam in previous protocols, without any adverse effects.
Perfusion Imaging Agent Drugs that can be used to measure blood flow to the heart are called perfusion imaging agents. During the PET scanning session, you may receive one or more injections of a positron-emitting perfusion imaging agent called [13N]-ammonia. The dose of active drug is vanishingly small, and so you should feel nothing unusual due to administration of the perfusion imaging agent.
Tritiated Norepinephrine Norepinephrine that has a trace amount of radioactivity in the form of tritium (tritiated norepinephrine) may be given i.v. Although norepinephrine is a powerful drug, the dose you will receive will be too small to produce any noticeable effects. Radiation doses to all organs are also very small, as explained below.
Another means to stimulate the sympathetic nervous system is LBNP. In this procedure, the legs and pelvic area are enclosed in an air-tight barrel-shaped chamber, and air is sucked out of the chamber, producing negative pressure. Blood pools in the legs, producing "artificial gravity". As during normal standing, the sympathetic nervous system is activated during LBNP. You may undergo LBNP at a mild intensity (15-25 millimeters of mercury of suction) during the PET scanning or in testing on a separate day.
We have used exercise, video games' or LBNP in several other clinical protocols, without any serious or long-lasting adverse effects.
If cardiac catheterization is already planned in your case in order to evaluate a heart disease, the research aspect of the cardiac catheterization involves about one hour extra in catheterization laboratory. The cardiac catheterization will be done in a special catheterization suite by a cardiologist with extensive experience in this procedure. Before the catheterization. you will be admitted to a cardiology ward, and you will be transported to the catheterization suite. After cleansing and numbing of the skin, a plastic catheter will be inserted into a vein in the arm or thigh. The catheter will be long enough that it can be threaded up the vein into the heart. The placement of the catheter will be verified by fluoroscopy, a type of real-time X-ray. Another catheter will be placed into an artery, by a procedure similar to that described above for he PET scanning.
During the cardiac catheterization, we will be measuring the rate of release of norepinephrine in the heart, by infusing a tiny amount of radioactive, tracer-labeled norepinephrine ([3H]-l-norepinephrine) and drawing blood samples from the vein draining your heart by way of a specially placed catheter. You will not notice anything unusual due to the infusion. It is possible that another i.Y. Will be placed, into which the tracer-labeled norepinephrine will be given.
During the infusion of tracer-labeled norepinephrine, you may receive one of the drugs described above, such as yohimbine, or you may play a video game. The infusion of the tracer labeled norepinephrine will last 20-60 minutes, depending on whether you undergo these other manipulations.
You may be asked to undergo re-testing under this protocol, such as after treatment with a drug. This would be done only with your additional consent at that time.
In addition to radiation exposure from positron-emitting fluorodopamine ([18F]-6F-DA), and radiation exposure from germanium-gallium (68Ge-68Ga, used for the preliminary transmission scanning before [18F]-6F-DA and before the perfusion imaging agent), you may undergo radiation exposure from tritiated norepinephrine ([3H]-l-NE, up to 100 microCuries), from the perfusion imaging agent. positron-emitting ammonia ([13N]-ammonia, [13N] NH3, 20 milliCuries of [13N]-ammonia per injection), or from fluoroscopy, which is used during cardiac catheterization to determine the positioning of the catheters. The following charts show the estimated radiation doses. The doses circled in the charts refer to the estimates in your case.
| [18F]-6F-DA | 68Ge-68Ga | [13N] NH3 | [3H]-l-NE | Fluoroscopy | |
|---|---|---|---|---|---|
| Highest dose | Bladder 0.8 | Heart 0.1 | Bladder 0.2 | Bladder 0.0 | Lungs 0.2 |
| 2d Highest | Kidneys 0.7 | Lungs 0.1 | Kidneys 0.1 | Kidneys 0.0 | Heart 0.1 |
| 3d Highest | Spleen 0.1 | Breast 0.1 | Brain 0.1 | Spleen 0.0 | Breast 0.0 |
| [18F]-6F-DA | 68Ge-68Ga | [13N] NH3 | [3H]-l-NE | Fluoroscopy | |
|---|---|---|---|---|---|
| Highest dose | Bladder 3.3 | Heart 0.1 | Bladder 0.6 | Bladder 0.0 | Lungs 0.2 |
| 2d Highest | Kidneys 2.9 | Lungs 0.1 | Kidneys 0.3 | Kidneys 0.0 | Heart 0.1 |
| 3d Highest | Spleen 0.6 | Breast 0.1 | Brain 0.3 | Spleen 0.0 | Breast 0.0 |
Please be aware that this radiation exposure is necessary for this research study only and is not essential for your medical care. The NIH Radiation Safety Committee and the NIH Radioactive Drug Research Committee have reviewed the use of radiation in this study and approved this use as being necessary to obtain the research information desired. For your reference, NIH Radiation Safety guidelines limit radiation exposure to 3 rems to any organ within a 13-week period and 5 rems annually; FDA regulatory limits are 5 rems per single administration and 15 rems per year to the above-listed organs.
The potential long-term risks from these radiation doses are uncertain, but these doses have never been associated with any definite adverse effects. Thus, the risk to you, if any, is estimated to be very slight.
The radiopharmaceuticals that you will receive are being administered under an Investigational New Drug (IND) approval from the Food and Drug Administration (FDA), with W. Eckelman, Ph.D., as the sponsor; both the sponsor and the FDA have access to the medical records of research subjects.
Please advise us if you have participated in research studies that involved use of radiation so that we can ensure that the total radiation dose from all studies is not excessive. Examples of such studies include x-ray studies in radiology departments, cardiac catheterization, fluoroscopy, and nuclear medicine studies (e.g., technetium and PET scans).
LBNP produces "artificial gravity". The amount of LBNP you will receive would correspond to less than the gravitational force you would undergo by simply standing up. LBNP should therefore be safe. One patient fainted during LBNP. The patient regained consciousness rapidly after LBNP was stopped. If you have a history of fainting, you may be at increased for fainting during LBNP. Your blood pressure will be monitored continuously, and if you have signs or symptoms suggesting that you may faint, LBNP will be stopped.
Headaches after lumbar punctures are due to the reduced amount of CSF. In most people, the CSF which is removed is rapidly replaced by the body. Prolonged headaches are thought to result from a persistent leakage of CSF from the area of the lumbar puncture. If your headache is prolonged, you and your doctor may decide to perform a "blood patch." A blood patch requires removing blood with a needle from a vein in your arm and then injecting it into the area of your back where the lumbar puncture was performed. This is done to seal the leak of CSF. Other rare complications of lumbar puncture include temporary double vision and infection.
As in all NIH clinical protocols, you may end your participation at any time, for any season.
| PET Scanning Procedures | ||||
|---|---|---|---|---|
| PET scanning (Chest, Abdomen, Limb) | Yes | No | ||
| Multiple blood samples | Yes | No | ||
| Arterial catheter | Yes | No | ||
| 24-hour urine collection | Yes | No | ||
| Inpatient observation | Yes | No | ||
| Physiological manipulations during PET scanning session | ||||
| Exercise | Yes | No | ||
| Video game | Yes | No | ||
| Lower body negative pressure (LBNP) | Yes | No | ||
| Drugs during PET scanning session | ||||
| 6-[18F]Fluorodopamine (radioactive) | Yes | No | ||
| [13N]-Ammonia (radioactive) | Yes | No | ||
| [3H]-l-Norepinephrine (radioactive) | Yes | No | ||
| Desiprarnine | Yes | No | ||
| Trimethaphan | Yes | No | ||
| Yohimbine | Yes | No | ||
| Nitroprusside | Yes | No | ||
| Methylprednisolone | Yes | No | ||
| Tyramine | Yes | No | ||
| Sedative | Yes | No | ||
| Associated Procedures (Different Day from PET Scanning) | ||||
|---|---|---|---|---|
| Magnetic resonance imaging (MRI) | Yes | No | ||
| Multiple blood samples | Yes | No | ||
| Arterial catheter | Yes | No | ||
| 24-hour urine collection | Yes | No | ||
| Inpatient observation | Yes | No | ||
| Physiological manipulations | ||||
| Exercise | Yes | No | ||
| Video game | Yes | No | ||
| Lower body negative pressure (LBNP) | Yes | No | ||
| Drugs | ||||
| [3H]-l-Norepinephrine (radioactive) | Yes | No | ||
| Desipramine | Yes | No | ||
| Trimethaphan | Yes | No | ||
| Yohimbine | Yes | No | ||
| Nitroprusside | Yes | No | ||
| Tyramine | Yes | No | ||
| Sedative | Yes | No | ||
| Lumbar puncture | Yes | No | ||
| Direct sympathetic nerve recording (92-N-144) | Yes | No | ||
| Right heart catheterization | Yes | No | ||
I have read the explanation about this study and have been given the opportunity to discuss it and to ask questions. I hereby consent to take part in this study.
Signature of Adult Patient & Date Signed
Signature of Investigator & Date Signed
B. Parent's Permission for Minor Patient.
I have read the explanation about this study and have been given the opportunity to discuss it and to ask questions. I hereby give permission for my child to take part in this study. (Attach NIH 2514-2, Minor's Assent, if applicable.)
Signature of Parent(s) & Date Signed
(If otherthan parent specify relationship)
Signature d Witness & Date Signed